lambda ladders promega Search Results


promega-markers lambda ladders  (Promega)
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Promega promega-markers lambda ladders
Promega Markers Lambda Ladders, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda ladder promega  (Promega)
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Promega lambda ladder promega
Lambda Ladder Promega, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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λ ladders  (Promega)
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Promega λ ladders
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
λ Ladders, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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markers lambda ladders  (Promega)
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Promega markers lambda ladders
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
Markers Lambda Ladders, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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promega-markers® lambda ladders marker  (Promega)
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Promega promega-markers® lambda ladders marker
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
Promega Markers® Lambda Ladders Marker, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda dna/hindiii dna ladder  (Promega)
90
Promega lambda dna/hindiii dna ladder
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
Lambda Dna/Hindiii Dna Ladder, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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molecular weight marker promega-markers lambda ladders  (Promega)
90
Promega molecular weight marker promega-markers lambda ladders
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
Molecular Weight Marker Promega Markers Lambda Ladders, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda hind iii ladder  (Promega)
90
Promega lambda hind iii ladder
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
Lambda Hind Iii Ladder, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda hindiii ladder  (Promega)
90
Promega lambda hindiii ladder
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
Lambda Hindiii Ladder, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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size marker lambda ladders 0.05-1 mb  (Promega)
90
Promega size marker lambda ladders 0.05-1 mb
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
Size Marker Lambda Ladders 0.05 1 Mb, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phage lambda dna hind iii/ eco ri digested ladder  (Promega)
90
Promega phage lambda dna hind iii/ eco ri digested ladder
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
Phage Lambda Dna Hind Iii/ Eco Ri Digested Ladder, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda ladder pfge markers 50--1000 kb  (Promega)
90
Promega lambda ladder pfge markers 50--1000 kb
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
Lambda Ladder Pfge Markers 50 1000 Kb, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of λ ladders (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .

Journal: Communications Biology

Article Title: DNA replication machinery prevents Rad52-dependent single-strand annealing that leads to gross chromosomal rearrangements at centromeres

doi: 10.1038/s42003-020-0934-0

Figure Lengend Snippet: a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of λ ladders (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .

Article Snippet: Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of λ ladders (Promega). c PCR analysis of GCR products recovered from agarose gels.

Techniques: Translocation Assay, Clone Assay, Staining, Amplification, Agarose Gel Electrophoresis, Electrophoresis